Detection of N. meningitidis group B antigens by MB-Dot-ELISA in patients with meningitis.

نویسندگان

  • G Alkmin M das
  • I M Landgraf
  • S H Shimizu
چکیده

Infection with Neisseria meningitidis group B has been difficult to detect, partly because this bacterial group's polysaccharide is a weak immunogen. This article describes work carried out to test a new procedure (MB-Dot-ELISA) employing a high-titered horse antiserum for detection of N. meningitidis group B antigens. The study assayed cerebrospinal fluid samples from 585 subjects, 574 with suspected meningitis cases and 11 with neurologic disorders. The results of the assay indicated a sensitivity of 0.991 and a specificity of 0.826. These results were superior to those obtained with latex agglutination and in substantial agreement with the results of counterimmunoelectrophoresis and bacteriologic methods. Overall, the MB-Dot-ELISA was found to be sensitive, inexpensive, and suitable for public health laboratory investigations.

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عنوان ژورنال:
  • Bulletin of the Pan American Health Organization

دوره 30 3  شماره 

صفحات  -

تاریخ انتشار 1996